The Experimental and Translational Immunomics Research Group, established in July 2012, conducts multidisciplinary research in the fields of immunology and genomics, with most of its efforts focused on the analysis of CD8+ T cell homing, immigration of CD8+ T cells into distinct tissue environments, phenotypic plasticity of Cd8+ T cells in different tissues, and regional immunity maintained by CD8+ T cells. The group investigates these questions using automated isolation of tissue-resident CD8+ T memory and effector cells, genomic scale description of their organ-specific characteristics, and tracking in vivo T cell activity and movement, using both transgenic mouse models and human clinical samples.
The research group typically uses the following methods and approaches :
Murine experimental acute GvHD model (Actm-OVA/OT-I)
Human patiensts diagnosed with acute GvHD
Murine experimental H1N1 flu infection model (A/PR/8/34)
Adoptive T cell transfer
In vivo T cell tracking
Automated tissue dissociation
Automated MACS sorting
Whole genome gene expression profiling
In vitro CD8+ T cell acivation
In vitro CD8+ T cell killing assay
Research infrastructure, equipment, transgenic mouse strains
Sufficient wet lab space, an automated tissue dissociation platform (Miltenyi Biotec gentleMACS Octo with Heating Upgrade), an automated magnetic cell sorting system (Miltenyi Biotec AutoMACS Pro), multiple BSL2 and BSL1 laminar flow hoods (Esco), a refrigerated Eppendorf centrifuge (Heraeus Fresco 17), desktop centrifuge (Heraeus Megafuge 16R), and sufficient storage capacity (liquid N2 tank, -80C freezer, -20C freezers, 4C fridges) are readily available. Besides, the group maintains several transgenic mouse strains, as follows (all on the C57Bl/6 backround): OT-I, Act-mOVA, CD45.1, UBC-GFP. Potential collaborators are encouraged to contact the group leader; we have several ongoing collaborations using the above infrastructure.
|Dr. Zoltán Pós, Associate Professor|
|Nikolett Lupsa, Research Fellow|
|Dr. Barbara Molnár-Érsek, Senior Research Fellow|
Melanoma-associated fibroblasts impair CD8+ T cell function and modify expression of immune checkpoint regulators via increased arginase activity.
Érsek B, Silló P, Cakir U, Molnár V, Bencsik A, Mayer B, Mezey E, Kárpáti S, Pós Z, Németh K.
Cell Mol Life Sci. 2020 Apr 23. doi: 10.1007/s00018-020-03517-8. PMID: 32328671