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About the group

 


The Experimental and Translational Immunomics Research Group, established in July 2012, conducts multidisciplinary research in the fields of immunology and genomics, with most of its efforts focused on the analysis of CD8+ T cell homing, immigration of CD8+ T cells into distinct tissue environments, phenotypic plasticity of Cd8+ T cells in different tissues, and regional immunity maintained by CD8+ T cells. The group investigates these questions using automated isolation of tissue-resident CD8+ T memory and effector cells, genomic scale description of their organ-specific characteristics, and tracking in vivo T cell activity and movement, using both transgenic mouse models and human clinical samples.   

  

Applied methodology 

The research group typically uses the following methods and approaches :

Murine experimental acute GvHD model (Actm-OVA/OT-I)

Human patiensts diagnosed with acute GvHD

Murine experimental H1N1 flu infection model (A/PR/8/34)

Adoptive T cell transfer

In vivo T cell tracking

Automated tissue dissociation

Automated MACS sorting 

FACS sorting

Whole genome gene expression profiling

Flow citometry

ELISA

ELISPOT

Q-PCR

IHC

TREC assay

Western blotting

In vitro CD8+ T cell acivation

In vitro CD8+ T cell killing assay

Research infrastructure, equipment, transgenic mouse strains 

Sufficient wet lab space, an automated tissue dissociation platform (Miltenyi Biotec gentleMACS Octo with Heating Upgrade), an automated magnetic cell sorting system (Miltenyi Biotec AutoMACS Pro), multiple BSL2 and BSL1 laminar flow hoods (Esco), a refrigerated Eppendorf centrifuge (Heraeus Fresco 17), desktop centrifuge (Heraeus Megafuge 16R), and sufficient storage capacity (liquid N2 tank, -80C freezer, -20C freezers, 4C fridges) are readily available. Besides, the group maintains several transgenic mouse strains, as follows (all on the C57Bl/6 backround): OT-I, Act-mOVA, CD45.1, UBC-GFP. Potential collaborators are encouraged to contact the group leader; we have several ongoing collaborations using the above infrastructure.

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Members

Head:

  PosZ Dr. Zoltán Pós, Associate Professor 

Members:

  BencsikA András Bencsik, PhD Student
  LupsaN Nikolett Lupsa, Research Fellow
  ErsekB Dr. Barbara Molnár-Érsek, Senior Research Fellow

Research Students:

  CzovekK Bence Hőbör
  CzovekK Ákos Szegedi
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Publications

 

 

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