About the group

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The Experimental and Translational Immunomics Research Group, established in July 2012, conducts multidisciplinary research in the fields of immunology and genomics, with most of its efforts focused on the analysis of CD8+ T cell homing, immigration of CD8+ T cells into distinct tissue environments, phenotypic plasticity of Cd8+ T cells in different tissues, and regional immunity maintained by CD8+ T cells. The group investigates these questions using automated isolation of tissue-resident CD8+ T memory and effector cells, genomic scale description of their organ-specific characteristics, and tracking in vivo T cell activity and movement, using both transgenic mouse models and human clinical samples.   

  

Applied methodology 

The research group typically uses the following methods and approaches :

Murine experimental acute GvHD model (Actm-OVA/OT-I)

Human patiensts diagnosed with acute GvHD

Murine experimental H1N1 flu infection model (A/PR/8/34)

Adoptive T cell transfer

In vivo T cell tracking

Automated tissue dissociation

Automated MACS sorting 

FACS sorting

Whole genome gene expression profiling

Flow citometry

ELISA

ELISPOT

Q-PCR

IHC

TREC assay

Western blotting

In vitro CD8+ T cell acivation

In vitro CD8+ T cell killing assay

Research infrastructure, equipment, transgenic mouse strains 

Sufficient wet lab space, an automated tissue dissociation platform (Miltenyi Biotec gentleMACS Octo with Heating Upgrade), an automated magnetic cell sorting system (Miltenyi Biotec AutoMACS Pro), multiple BSL2 and BSL1 laminar flow hoods (Esco), a refrigerated Eppendorf centrifuge (Heraeus Fresco 17), desktop centrifuge (Heraeus Megafuge 16R), and sufficient storage capacity (liquid N2 tank, -80C freezer, -20C freezers, 4C fridges) are readily available. Besides, the group maintains several transgenic mouse strains, as follows (all on the C57Bl/6 backround): OT-I, Act-mOVA, CD45.1, UBC-GFP. Potential collaborators are encouraged to contact the group leader; we have several ongoing collaborations using the above infrastructure.

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Members

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Head:

Members:

PhD Students:

 

Research Students:

  • Anett Tóth
  • Eszter Sarzsinszky
  • Andor Horváth

 

 

 

 


Publications

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Unique patterns of CD8+ T-cell-mediated organ damage in the Act-mOVA/OT-I model of acute graft-versus-host disease Érsek BLupsa N, Pócza P, Tóth AHorváth A, Molnár V, Bagita B, Bencsik A, Hegyesi H, Matolcsy A, Buzás EI, Pós ZCell Mol Life Sci. 2016 Oct;73(20):3935-47. doi: 10.1007/s00018-016-2237-7. PMID: 27137185

High-dimensional analysis of the aging immune system: verification of age-associated differences in immune signaling responses in healthy donors. Longo DM, Louie B, Ptacek J, Friedland G, Evensen E, Putta S, Atallah M, Spellmeyer D, Wang E, Pos Z, Marincola FM, Schaeffer A, Lukac S, Railkar R, Beals CR, Cesano A, Carayannopoulos LN, Hawtin RE. J Transl Med. 2014 Jun 21;12:178. doi: 10.1186/1479-5876-12-178. PMID:24952610

Differential Responses of Plasmacytoid Dendritic Cells to Influenza Virus and Distinct Viral Pathogens Thomas JM, Pos Z, Reinboth J, Wang RY, Wang E, Frank GM, Lusso P, Trinchieri G, Alter HJ, Marincola FM, Thomas E. J Virol. 2014 Sep;88(18):10758-66. doi: 10.1128/JVI.01501-14. PMID: 25008918

Global analyses of human immune variation reveal baseline predictors of postvaccination responses. Tsang JS, Schwartzberg PL, Kotliarov Y, Biancotto A, Xie Z, Germain RN, Wang E, Olnes MJ, Narayanan M, Golding H, Moir S, Dickler HB, Perl S, Cheung F; Collaborators: Obermoser G, Chaussabel D, Palucka K, Chen J, Fuchs JC, Ho J, Khurana S, King LR, Langweiler M, Liu H, Manischewitz J, Pos Z, Posada JG, Schum P, Shi R, Valdez J, Wang W, Zhou H, Kastner DL, Marincola FM, McCoy JP, Trinchieri G, Young NS. Cell. 2014 Apr 10;157(2):499-513. doi: 10.1016/j.cell.2014.03.031.PMID: 24725414

Melanoma NOS1 expression promotes dysfunctional IFN signaling. Liu Q, Tomei S, Ascierto ML, De Giorgi V, Bedognetti D, Dai C, Uccellini L, Spivey T, Pos Z, Thomas J, Reinboth J, Murtas D, Zhang Q, Chouchane L, Weiss GR, Slingluff CL Jr, Lee PP, Rosenberg SA, Alter H, Yao K, Wang E, Marincola FM. J Clin Invest. 2014 Apr 1. pii: 69611. doi: 10.1172/JCI69611. [Epub ahead of print] PMID:24691438

Longitudinal study of recurrent metastatic melanoma cell lines underscores the individuality of cancer biology. Pos Z, Spivey TL, Liu H, Sommariva M, Chen J, Wunderlich JR, Parisi G, Tomei S, Ayotte BD, Stroncek DF, Malek JA, Robbins PF, Rivoltini L, Maio M, Chouchane L, Wang E, Marincola FM. J Invest Dermatol. 2014 May;134(5):1389-96. doi: 10.1038/jid.2013.495. Epub 2013 Nov 22. PMID:24270663

RDH10, RALDH2, and CRABP2 are required components of PPARγ-directed ATRA synthesis and signaling in human dendritic cells. Gyöngyösi A, Szatmari I, Pap A, Dezso B, Pos Z, Széles L, Varga T, Nagy L. J Lipid Res. 2013 Sep;54(9):2458-74. doi: 10.1194/jlr.M038984. Epub 2013 Jul 6. PMID: 23833249

 

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