About the group

 

The Buzás lab contributed to the extracellular vesicle field with the following observations:

  1. Demonstration that extracellular vesicles and protein aggregates share several biophysical parameters, and thus, protein aggregates may interfere with extracellular vesicle detection by flow cytometry and may contaminate vesicle preparations (http://www.ncbi.nlm.nih.gov/pubmed/21041717);
  2. Demonstration that extracellular vesicles and protein aggregates may be discriminated by differential detergent lysis (http://www.ncbi.nlm.nih.gov/pubmed/21041717);
  3. Introduction of gravity driven size filtration to extracellular vesicle isolation (http://www.ncbi.nlm.nih.gov/pubmed/21041717);
  4. Demonstration that the use of ACD anticoagulant tubes prevents artefactual in vitro platelet vesiculation (http://www.ncbi.nlm.nih.gov/pubmed/24360116);
  5. Identification of disease specific extracellular vesicle signature in the synovial fluid of patients with various joint diseases (http://www.ncbi.nlm.nih.gov/pubmed/23185418);
  6. Demonstration that extracellular vesicles have combinatorial effect with cytokines on the gene expression of cells; (http://www.ncbi.nlm.nih.gov/pubmed/24705984)
  7. Introduction of a simple 96 well plate total lipid assay to the assessment of the lipid content of extracellular vesicles; introduction of protein to lipid ratio as a parameter characteristic of different extracellular vesicle subpopulations and as a quality control parameter of extracellular vesicles (http://www.ncbi.nlm.nih.gov/pubmed/25798862);
  8. Demonstration that different extracellular vesicle subpopulations are characterized by differential lipid properties exosomes being characterized by the highest lipid packing densities (http://www.ncbi.nlm.nih.gov/pubmed/25798862);
  9. Combination of tunable resistive pulse sensing with detergent lysis to prove the vesicular nature of particles and to determine the differential extracellular vesicle subpopulation lysing concentrations of different detergents.(http://www.ncbi.nlm.nih.gov/pubmed/26264754)
  10. First demonstration of the association of glycosidases with extracellular vesicles (http://www.ncbi.nlm.nih.gov/pubmed/23099419http://www.ncbi.nlm.nih.gov/pubmed/19442276)
  11. First demonstration that LDL mimicks exososmes and microvesicles during isolation and detection. Demondtration that LDL associates with extracellular vesicles in vitro. (https://www.nature.com/articles/srep24316.pdf)
  12. Demonstration that during monocyte activation cell surface thiols are preserved by the release of extracellular vesicles with oxidized membrane pathches (https://www.ncbi.nlm.nih.gov/pubmed/28323130)
  13. Development of a simple flow cytometry assay to determine the potency of pre-implantation embyos (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5216337/)
  14. First demonstration of antibotics-induced release of exosomes with surface-associated DNA (Németh A et a. Sci Rep 2017)

 

Methods used to isolate and characterize extracellular vesicles:

Sequential differential centrifugation/ultracentrifugation combined with gravity size filtration, sucrose gradient ultracentrifugation, sucrose cushion ultracentrifugation, OptiprepTM density gradient ultracentrifugation, size exclusion chromatography, ultrafiltration, affinity capture magnetic isolation of extracellular vesicles, flow cytometry (direct or bead bound), Western blotting, tunable resistive pulse sensing (also combined with differential detergent lysis), RNA/DNA/lipid/protein/glycan analysis, transmission electron microscopy, atomic force microscopy

 

Academic collaborations (name, institution, city, country, topic):

  • Ágnes Kittel, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary (electron microscopy of extracellular vesicles)
  • Róbert Horváth, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest (analysis of extracellular vesicles by label free optical biosensors)
  • Erzsébet Ligeti, Department of Physiology, Semmelweis University (neutrophil-derived extracellular vesicles)
  • Mária Deli Laboratory of Molecular Neurobiology, Institute of Biophysics, Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary (AFM of extracellular vesicles)
  • Mária Csete University of Szeged, Department of Optics and Quantum Electronics, Szeged (AFM of extracellular vesicles)
  • János Matkó, Andrea Balogh Department of Immunology, Eötvös Loránd University, Budapest, Hungary (spectroratiometric measurements of extracellular vesicles)
  • Mihály Józsi, Department of Immunology, Eötvös Lóránd University (extracellular vesicles and complement components)
  • Szigeti Krisztián Department of Biophysics and Radiation Biology, Semmelweis University, Budapest (biodistribution analysis of extracellular vesicles)
  • Zoltán Varga Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest (radiolabelling of extracellular vesicles for biodistribution analysis)
  • Károly Vékey, Lilla Turiák-Research Centre for Natural Sciences, Hungarian (MS analysis of extracellular vesicles)
  • Zoltán Giricz, Péter FerdinándyDepartment of Pharmacology and Pharmacotherapy, Semmelweis University, Budapest (characterization of blood plasma extracellular vesicles mediating remote ischemic preconditioning)
  • Katalin Kristóf, Dóra Szabó Department of Microbiology, Semmelweis University, Budapest (production and immune recognition of bacterial extracellular vesicles)

 

International collaborations: 

  • Steffen Gay, Center of Experimental Rheumatology, University Hospital Zurich and Zurich Center of Integrative Human Physiology
  • Bence György, The Massachusetts General Hospital, and Neuroscience Program, Harvard Medical School, Boston
  • Hans Joachim Gabius, Faculty of Veterinary Medicine, Ludwig-Maximilians-University Munich (analysis of galectin SNP sin RA and OA)
  • Jan Lötwall, University of Gothenburg, Sweden (analysis of RNA in different subpopulations of extracellular vesicles)
  • Marca Wauben, Department of Biochemistry & Cell Biology, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands (high resolution flow cytometry for single particle analysis of exosomes)
  • Dolores di Vizio, Division of Cancer Biology and Therapeutics, Cedars-Sinai Medical Center, Los Angeles, CA, USA (comparative analysis of large oncosomes and apoptotic bodies)
  • Dolores Solis, Instituto de Química Física Rocasolano, Consejo Superior de Investigaciones Científicas, Madrid, Spain

 

Clinical collaborations (name, institution, city, country, topic):

  • Béla Merkely, Annamária Kosztin, Tamás Radovits, Heart and Vascular Center, Semmelweis University, Budapest (analysis of extracellular vesicles in the pericardial fluid samples of CABG patients as well as heart transplantation donors and recipients
  • László Harsányi, Ákos Szűcs, 1st Department of Surgery, Semmelweis University, Budapes (analysis of extracellular vesicles in pancreatic juice )
  • János Rigó, First Department of Obstetrics and Gynecology, Semmelweis University, Budapest (analysis of extracellular vesicles in blood plasma samples of normal pregnants as well as preeclamptic pregnants)
  • György Nagy, Polyclinic of the Hospitaller Brothers of St. John of God in Budapest (analysis of extracellular vesicles in early rheumatoid arthritis)
  • Anna Polgár, National Institute of Rheumatology and Physiotherapy, Budapest (analysis of extracellular vesicles in early rheumatoid arthritis)

 

Industrial collaborations (name, institution, city, country, topic):

  • Attana, Stockholm, Sweden (analysis of surface lectin binding properties of extracellular vesicles by quartz crystal microbalance (QCM))

 

Members

Leader:


  BuzasE

Prof. Edit Buzás, Professor and Chairman

 

Members:


  Bekes Marta Márta Békés, PhD Student
(Supervisor: Dr. Viola Tamási)
  Fekete Nora Nóra Fekete, Assistant 
  ForsonitsA András Försönits, PhD Student
(Supervisor: Dr. Edit Buzás)
  SzaboK Dr. Katalin Szabó-Taylor, Assistant Lecturer 
  Kovacs Arpad Dr. Árpád Kovács, PhD Student 
(Supervisor: Dr. Éva Pállinger)
  PapE Dr. Erna Pap, Associate Professor
  Oszvald Adam Ádám Oszvald, PhD Student
(Supervisors: Dr. Edit Buzás, Dr. Zoltán Wiener)
  PallingerE

Dr. Éva Pállinger, Associate Professor

  PalocziK Krisztina Pálóczi, Technical Assistant
  Tamasi Viola

Dr. Viola Tamási, Associate Professor

  TothS Dr. Sára Tóth, Associate Professor
  Toth Eszter Eszter Tóth, PhD Student
(Supervisors: Dr. Edit Buzás, Dr. Katalin Szabó-Taylor)
  Varga Anita Anita Varga, PhD Student
(Supervisor: Dr. Edit Buzás)
  Visnovitz Tamas

Dr. Tamás Visnovitz, Research Fellow

  VukmanK Dr. Krisztina Vukman, Research Fellow
  Sodar Barbara

Dr. Barbara Sódar, Research Assistant

 

 

Publications

2017

 

2016

 

2015
  • Applying extracellular vesicles based therapeutics in clinical trials - an ISEV position paper.
    Lener T, Gimona M, Aigner L, Börger V, Buzas E, Camussi G, Chaput N, Chatterjee D, Court FA, Del Portillo HA, O'Driscoll L, Fais S, Falcon-Perez JM, Felderhoff-Mueser U, Fraile L, Gho YS, Görgens A, Gupta RC, Hendrix A, Hermann DM, Hill AF, Hochberg F, Horn PA, de Kleijn D, Kordelas L, Kramer BW, Krämer-Albers EM, Laner-Plamberger S, Laitinen S, Leonardi T, Lorenowicz MJ, Lim SK, Lötvall J, Maguire CA, Marcilla A, Nazarenko I, Ochiya T, Patel T, Pedersen S, Pocsfalvi G, Pluchino S, Quesenberry P, Reischl IG, Rivera FJ, Sanzenbacher R, Schallmoser K, Slaper-Cortenbach I, Strunk D, Tonn T, Vader P, van Balkom BW, Wauben M, Andaloussi SE, Théry C, Rohde E, Giebel B.
    J Extracell Vesicles. 2015 Dec 31;4:30087. doi: 10.3402/jev.v4.30087. eCollection 2015.

  • Isolation of Exosomes from Blood Plasma: Qualitative and Quantitative Comparison of Ultracentrifugation and Size Exclusion Chromatography Methods.
    Baranyai T, Herczeg K, Onódi Z, Voszka I, Módos K, Marton N, Nagy G, Mäger I, Wood MJ, El Andaloussi S, Pálinkás Z, Kumar V, Nagy P, Kittel Á, Buzás EI, Ferdinandy P, Giricz Z.
    PLoS One. 2015 Dec 21;10(12):e0145686. doi: 10.1371/journal.pone.0145686. eCollection 2015.

  • Differential detergent sensitivity of extracellular vesicle subpopulations.
    Osteikoetxea X, Sódar B, Németh A, Szabó-Taylor K, Pálóczi K, Vukman KV, Tamási V, Balogh A, Kittel Á, Pállinger É, Buzás EI.
    Org Biomol Chem2015 Sep 23;13(38):9775-82. doi: 10.1039/c5ob01451d.

  • Biological properties of extracellular vesicles and their physiological functions.
    Yáñez-Mó M, Siljander PR, Andreu Z, Zavec AB, Borràs FE, Buzas EI, Buzas K, Casal E, Cappello F, Carvalho J, Colás E, Cordeiro-da Silva A, Fais S, Falcon-Perez JM, Ghobrial IM, Giebel B, Gimona M, Graner M, Gursel I, Gursel M, Heegaard NH, Hendrix A, Kierulf P, Kokubun K, Kosanovic M, Kralj-Iglic V, Krämer-Albers EM, Laitinen S, Lässer C, Lener T, Ligeti E, Linē A, Lipps G, Llorente A, Lötvall J, Manček-Keber M, Marcilla A, Mittelbrunn M, Nazarenko I, Nolte-'t Hoen EN, Nyman TA, O'Driscoll L, Olivan M, Oliveira C, Pállinger É, Del Portillo HA, Reventós J, Rigau M, Rohde E, Sammar M, Sánchez-Madrid F, Santarém N, Schallmoser K, Ostenfeld MS, Stoorvogel W, Stukelj R, Van der Grein SG, Vasconcelos MH, Wauben MH, De Wever O.
    J Extracell Vesicles2015 May 14;4:27066. doi: 10.3402/jev.v4.27066. eCollection 2015.

  • Improved characterization of EV preparations based on protein to lipid ratio and lipid properties.
    Osteikoetxea X, Balogh A, Szabó-Taylor K, Németh A, Szabó TG, Pálóczi K, Sódar B, Kittel Á, György B, Pállinger É, Matkó J, Buzás EI.
    PLoS One2015 Mar 23;10(3):e0121184. doi: 10.1371/journal.pone.0121184. eCollection 2015.

  • Oxidative and other posttranslational modifications in extracellular vesicle biology.
    Szabó-Taylor K, Ryan B, Osteikoetxea X, Szabó TG, Sódar B, Holub M, Németh A, Pálóczi K, Pállinger É, Winyard P, Buzás EI.
    Semin Cell Dev Biol2015 Apr;40:8-16. doi: 10.1016/j.semcdb.2015.02.012. Epub 2015 Feb 24. Review.

  • Mass spectrometry of extracellular vesicles.
    Pocsfalvi G, Stanly C, Vilasi A, Fiume I, Capasso G, Turiák L, Buzas EI, Vékey K.
    Mass Spectrom Rev2015 Feb 20. doi: 10.1002/mas.21457. [Epub ahead of print]

 
2014
 
2013
 
2012
  • Kalra H, Simpson RJ, Ji H, Aikawa E, Altevogt P, Askenase P, Bond VC, Borras FE, Breakefield X, Budnik V, Buzas E, Camussi G, Clayton A, Cocucci E, Falcon-Perez JM, Gabrielsson S, Gho YS, Gupta D, Harsha HC, Hendrix A, Hill AF, Inal JM, Jenster G, Krämer-Albers EM, Lim SK, Llorente A, Lötvall J, Marcilla A, Mincheva-Nilsson L, Nazarenko I, Nieuwland R, Nolte-'t Hoen EN, Pandey A, Patel T, Piper MG, Pluchino S, Prasad TS, Rajendran L, Raposo G, Record M, Reid GE, Sánchez-Madrid F, Schiffelers RM, Siljander P, Stensballe A, Stoorvogel W, Taylor D, Thery C, Valadi H, van Balkom BW, Vázquez J, Vidal M, Wauben MH, Yánez-Mó M, Zoeller M, Mathivanan S.
    PLoS Biol. 2012 Dec;10(12):e1001450. Epub 2012 Dec 18.
  • György B, Szabó TG, Turiák L, Wright M, Herczeg P, Lédeczi Z, Kittel A, Polgár A, Tóth K, Dérfalvi B, Zelenák G, Böröcz I, Carr B, Nagy G, Vékey K, Gay S, Falus A, Buzás EI. 
    PLoS One. 2012; 7(11): e49726. Epub 2012 Nov 20.
 
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Other publications

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