The Buzás lab contributed to the extracellular vesicle field with the following observations:
Methods used to isolate and characterize extracellular vesicles:
Sequential differential centrifugation/ultracentrifugation combined with gravity size filtration, sucrose gradient ultracentrifugation, sucrose cushion ultracentrifugation, OptiprepTM density gradient ultracentrifugation, size exclusion chromatography, ultrafiltration, affinity capture magnetic isolation of extracellular vesicles, flow cytometry (direct or bead bound), Western blotting, tunable resistive pulse sensing (also combined with differential detergent lysis), RNA/DNA/lipid/protein/glycan analysis, transmission electron microscopy, atomic force microscopy
Academic collaborations (name, institution, city, country, topic):
International collaborations:
Clinical collaborations (name, institution, city, country, topic):
Industrial collaborations (name, institution, city, country, topic):
Prof. Edit Buzás, Professor and Chairman
|
Nóra Fekete, Assistant |
András Försönits, PhD Student |
Alicia Galinsoga, PhD Student |
Dr. Hargita Hegyesi, Associate Professor |
Delaram Khamari, PhD Student |
Anna Koncz, PhD Student |
Dr. Erna Pap, Associate Professor |
Dr. Éva Pállinger, Associate Professor |
Krisztina Pálóczi, Technical Assistant |
Gábor Seregélyes, PhD Student |
Dr. Katalin Szabó-Taylor, Assistant Lecturer |
Dr. Viola Tamási, Associate Professor |
Dr. Sára Tóth, Associate Professor |
Eszter Tóth, PhD Student |
Dr. Tamás Visnovitz, Research Fellow |
Dr. Krisztina Vukman, Research Fellow |
2017
Evaluation and diagnostic potential of circulating extracellular vesicle-associated microRNAs in adrenocortical tumors.
Perge P, Butz H, Pezzani R, Bancos I, Nagy Z, Pálóczi K, Nyírő G, Decmann Á, Pap E, Luconi M, Mannelli M, Buzás EI, Tóth M, Boscaro M, Patócs A, Igaz P.
Sci Rep. 2017 Jul 14;7(1):5474. doi: 10.1038/s41598-017-05777-0.
Microvesicles in vascular homeostasis and diseases. Position Paper of the European Society of Cardiology (ESC) Working Group on Atherosclerosis and Vascular Biology.
Ridger VC, Boulanger CM, Angelillo-Scherrer A, Badimon L, Blanc-Brude O, Bochaton-Piallat ML, Boilard E, Buzas EI, Caporali A, Dignat-George F, Evans PC, Lacroix R, Lutgens E, Ketelhuth DFJ, Nieuwland R, Toti F, Tunon J, Weber C, Hoefer IE.
Thromb Haemost. 2017 Jun 28;117(7):1296-1316. doi: 10.1160/TH16-12-0943. Epub 2017 Jun 1.
Methodological Guidelines to Study Extracellular Vesicles.
Coumans FAW, Brisson AR, Buzas EI, Dignat-George F, Drees EEE, El-Andaloussi S, Emanueli C, Gasecka A, Hendrix A, Hill AF, Lacroix R, Lee Y, van Leeuwen TG, Mackman N, Mäger I, Nolan JP, van der Pol E, Pegtel DM, Sahoo S, Siljander PRM, Sturk G, de Wever O, Nieuwland R.
Circ Res. 2017 May 12;120(10):1632-1648. doi: 10.1161/CIRCRESAHA.117.309417. Review.
Extracellular vesicles regulate the human osteoclastogenesis: divergent roles in discrete inflammatory arthropathies.
Marton N, Kovács OT, Baricza E, Kittel Á, Győri D, Mócsai A, Meier FMP, Goodyear CS, McInnes IB, Buzás EI, Nagy G.
Cell Mol Life Sci. 2017 May 10. doi: 10.1007/s00018-017-2535-8. [Epub ahead of print]
Obstacles and opportunities in the functional analysis of extracellular vesicle RNA - an ISEV position paper.
Mateescu B, Kowal EJ, van Balkom BW, Bartel S, Bhattacharyya SN, Buzás EI, Buck AH, de Candia P, Chow FW, Das S, Driedonks TA, Fernández-Messina L, Haderk F, Hill AF, Jones JC, Van Keuren-Jensen KR, Lai CP, Lässer C, Liegro ID, Lunavat TR, Lorenowicz MJ, Maas SL, Mäger I, Mittelbrunn M, Momma S, Mukherjee K, Nawaz M, Pegtel DM, Pfaffl MW, Schiffelers RM, Tahara H, Théry C, Tosar JP, Wauben MH, Witwer KW, Nolte-'t Hoen EN.
J Extracell Vesicles. 2017 Mar 7;6(1):1286095. doi: 10.1080/20013078.2017.1286095. eCollection 2017.
Monocyte activation drives preservation of membrane thiols by promoting release of oxidised membrane moieties via extracellular vesicles.
Szabó-Taylor KÉ, Tóth EÁ, Balogh AM, Sódar BW, Kádár L, Pálóczi K, Fekete N, Németh A, Osteikoetxea X, Vukman KV, Holub M, Pállinger É, Nagy G, Winyard PG, Buzás EI.
Free Radic Biol Med. 2017 Jul;108:56-65. doi: 10.1016/j.freeradbiomed.2017.03.016. Epub 2017 Mar 18.
Mast cell secretome: Soluble and vesicular components.
Vukman KV, Försönits A, Oszvald Á, Tóth EÁ, Buzás EI.
Semin Cell Dev Biol. 2017 Jul;67:65-73. doi: 10.1016/j.semcdb.2017.02.002. Epub 2017 Feb 9. Review.
European Network on Microvesicles and Exosomes in Health and Disease (ME-HaD).
O'Driscoll L, Stoorvogel W, Théry C, Buzas E, Nazarenko I, Siljander P, Yáñez-Mó M, Fais S, Giebel B, Yliperttula M.
Eur J Pharm Sci. 2017 Feb 15;98:1-3. doi: 10.1016/j.ejps.2017.01.003. No abstract available.
A simple and rapid flow cytometry-based assay to identify a competent embryo prior to embryo transfer.
Pallinger E, Bognar Z, Bodis J, Csabai T, Farkas N, Godony K, Varnagy A, Buzas E, Szekeres-Bartho J.
Sci Rep. 2017 Jan 6;7:39927. doi: 10.1038/srep39927.
Single particle analysis: Methods for detection of platelet extracellular vesicles in suspension (excluding flow cytometry).
Buzás EI, Gardiner C, Lee C, Smith ZJ.
Platelets. 2017 May;28(3):249-255. doi: 10.1080/09537104.2016.1260704. Epub 2016 Dec 29.
2016
The International Society for Extracellular Vesicles launches the first massive open online course on extracellular vesicles.
Lässer C, Théry C, Buzás EI, Mathivanan S, Zhao W, Gho YS, Lötvall J.
J Extracell Vesicles. 2016 Dec 16;5:34299. doi: 10.3402/jev.v5.34299. eCollection 2016.
A standardized method to determine the concentration of extracellular vesicles using tunable resistive pulse sensing.
Vogel R, Coumans FA, Maltesen RG, Böing AN, Bonnington KE, Broekman ML, Broom MF, Buzás EI, Christiansen G, Hajji N, Kristensen SR, Kuehn MJ, Lund SM, Maas SL, Nieuwland R, Osteikoetxea X, Schnoor R, Scicluna BJ, Shambrook M, de Vrij J, Mann SI, Hill AF, Pedersen S.
J Extracell Vesicles. 2016 Sep 27;5:31242. doi: 10.3402/jev.v5.31242. eCollection 2016.
The Role of Extracellular Vesicle and Tunneling Nanotube-Mediated Intercellular Cross-Talk Between Mesenchymal Stem Cells and Human Peripheral T Cells.
Matula Z, Németh A, Lőrincz P, Szepesi Á, Brózik A, Buzás EI, Lőw P, Német K, Uher F, Urbán VS.
Stem Cells Dev. 2016 Dec 1;25(23):1818-1832. Epub 2016 Oct 4.
[Extracellular vesicles and their role in hematological malignancies].
Rzepiel A, Kutszegi N, Cs Sági J, Kelemen A, Pálóczi K, F Semsei Á, Buzás E, Erdélyi DJ.
Orv Hetil. 2016 Aug;157(35):1379-84. doi: 10.1556/650.2016.30532. Review. Hungarian.
Immune Recognition of Citrullinated Proteoglycan Aggrecan Epitopes in Mice with Proteoglycan-Induced Arthritis and in Patients with Rheumatoid Arthritis.
Markovics A, Ocskó T, Katz RS, Buzás EI, Glant TT, Mikecz K.
PLoS One. 2016 Jul 28;11(7):e0160284. doi: 10.1371/journal.pone.0160284. eCollection 2016.
Radiolabeling of Extracellular Vesicles with (99m)Tc for Quantitative In Vivo Imaging Studies.
Varga Z, Gyurkó I, Pálóczi K, Buzás EI, Horváth I, Hegedűs N, Máthé D, Szigeti K.
Cancer Biother Radiopharm. 2016 Jun;31(5):168-73. doi: 10.1089/cbr.2016.2009.
Unique patterns of CD8+ T-cell-mediated organ damage in the Act-mOVA/OT-I model of acute graft-versus-host disease.
Érsek B, Lupsa N, Pócza P, Tóth A, Horváth A, Molnár V, Bagita B, Bencsik A, Hegyesi H, Matolcsy A, Buzás EI, Pós Z.
Cell Mol Life Sci. 2016 Oct;73(20):3935-47. doi: 10.1007/s00018-016-2237-7. Epub 2016 Apr 30.
Low-density lipoprotein mimics blood plasma-derived exosomes and microvesicles during isolation and detection.
Sódar BW, Kittel Á, Pálóczi K, Vukman KV, Osteikoetxea X, Szabó-Taylor K, Németh A, Sperlágh B, Baranyai T, Giricz Z, Wiener Z, Turiák L, Drahos L, Pállinger É, Vékey K, Ferdinandy P, Falus A, Buzás EI.
Sci Rep. 2016 Apr 18;6:24316. doi: 10.1038/srep24316.
Evidence-Based Clinical Use of Nanoscale Extracellular Vesicles in Nanomedicine.
Fais S, O'Driscoll L, Borras FE, Buzas E, Camussi G, Cappello F, Carvalho J, Cordeiro da Silva A, Del Portillo H, El Andaloussi S, Ficko Trček T, Furlan R, Hendrix A, Gursel I, Kralj-Iglic V, Kaeffer B, Kosanovic M, Lekka ME, Lipps G, Logozzi M, Marcilla A, Sammar M, Llorente A, Nazarenko I, Oliveira C, Pocsfalvi G, Rajendran L, Raposo G, Rohde E, Siljander P, van Niel G, Vasconcelos MH, Yáñez-Mó M, Yliperttula ML, Zarovni N, Zavec AB, Giebel B.
ACS Nano. 2016 Apr 26;10(4):3886-99. doi: 10.1021/acsnano.5b08015. Epub 2016 Mar 15.
Extracellular vesicles in cardiovascular disease: are they Jedi or Sith?
Osteikoetxea X, Németh A, Sódar BW, Vukman KV, Buzás EI.
J Physiol. 2016 Jun 1;594(11):2881-94. doi: 10.1113/JP271336. Epub 2016 Mar 20.
Applying extracellular vesicles based therapeutics in clinical trials - an ISEV position paper.
Lener T, Gimona M, Aigner L, Börger V, Buzas E, Camussi G, Chaput N, Chatterjee D, Court FA, Del Portillo HA, O'Driscoll L, Fais S, Falcon-Perez JM, Felderhoff-Mueser U, Fraile L, Gho YS, Görgens A, Gupta RC, Hendrix A, Hermann DM, Hill AF, Hochberg F, Horn PA, de Kleijn D, Kordelas L, Kramer BW, Krämer-Albers EM, Laner-Plamberger S, Laitinen S, Leonardi T, Lorenowicz MJ, Lim SK, Lötvall J, Maguire CA, Marcilla A, Nazarenko I, Ochiya T, Patel T, Pedersen S, Pocsfalvi G, Pluchino S, Quesenberry P, Reischl IG, Rivera FJ, Sanzenbacher R, Schallmoser K, Slaper-Cortenbach I, Strunk D, Tonn T, Vader P, van Balkom BW, Wauben M, Andaloussi SE, Théry C, Rohde E, Giebel B.
J Extracell Vesicles. 2015 Dec 31;4:30087. doi: 10.3402/jev.v4.30087. eCollection 2015.
Isolation of Exosomes from Blood Plasma: Qualitative and Quantitative Comparison of Ultracentrifugation and Size Exclusion Chromatography Methods.
Baranyai T, Herczeg K, Onódi Z, Voszka I, Módos K, Marton N, Nagy G, Mäger I, Wood MJ, El Andaloussi S, Pálinkás Z, Kumar V, Nagy P, Kittel Á, Buzás EI, Ferdinandy P, Giricz Z.
PLoS One. 2015 Dec 21;10(12):e0145686. doi: 10.1371/journal.pone.0145686. eCollection 2015.
Differential detergent sensitivity of extracellular vesicle subpopulations.
Osteikoetxea X, Sódar B, Németh A, Szabó-Taylor K, Pálóczi K, Vukman KV, Tamási V, Balogh A, Kittel Á, Pállinger É, Buzás EI.
Org Biomol Chem. 2015 Sep 23;13(38):9775-82. doi: 10.1039/c5ob01451d.
Biological properties of extracellular vesicles and their physiological functions.
Yáñez-Mó M, Siljander PR, Andreu Z, Zavec AB, Borràs FE, Buzas EI, Buzas K, Casal E, Cappello F, Carvalho J, Colás E, Cordeiro-da Silva A, Fais S, Falcon-Perez JM, Ghobrial IM, Giebel B, Gimona M, Graner M, Gursel I, Gursel M, Heegaard NH, Hendrix A, Kierulf P, Kokubun K, Kosanovic M, Kralj-Iglic V, Krämer-Albers EM, Laitinen S, Lässer C, Lener T, Ligeti E, Linē A, Lipps G, Llorente A, Lötvall J, Manček-Keber M, Marcilla A, Mittelbrunn M, Nazarenko I, Nolte-'t Hoen EN, Nyman TA, O'Driscoll L, Olivan M, Oliveira C, Pállinger É, Del Portillo HA, Reventós J, Rigau M, Rohde E, Sammar M, Sánchez-Madrid F, Santarém N, Schallmoser K, Ostenfeld MS, Stoorvogel W, Stukelj R, Van der Grein SG, Vasconcelos MH, Wauben MH, De Wever O.
J Extracell Vesicles. 2015 May 14;4:27066. doi: 10.3402/jev.v4.27066. eCollection 2015.
Improved characterization of EV preparations based on protein to lipid ratio and lipid properties.
Osteikoetxea X, Balogh A, Szabó-Taylor K, Németh A, Szabó TG, Pálóczi K, Sódar B, Kittel Á, György B, Pállinger É, Matkó J, Buzás EI.
PLoS One. 2015 Mar 23;10(3):e0121184. doi: 10.1371/journal.pone.0121184. eCollection 2015.
Oxidative and other posttranslational modifications in extracellular vesicle biology.
Szabó-Taylor K, Ryan B, Osteikoetxea X, Szabó TG, Sódar B, Holub M, Németh A, Pálóczi K, Pállinger É, Winyard P, Buzás EI.
Semin Cell Dev Biol. 2015 Apr;40:8-16. doi: 10.1016/j.semcdb.2015.02.012. Epub 2015 Feb 24. Review.
Mass spectrometry of extracellular vesicles.
Pocsfalvi G, Stanly C, Vilasi A, Fiume I, Capasso G, Turiák L, Buzas EI, Vékey K.
Mass Spectrom Rev. 2015 Feb 20. doi: 10.1002/mas.21457. [Epub ahead of print]
Minimal experimental requirements for definition of extracellular vesicles and their functions: a position statement from the International Society for Extracellular Vesicles.
Lötvall J, Hill AF, Hochberg F, Buzás EI, Di Vizio D, Gardiner C, Gho YS, Kurochkin IV, Mathivanan S, Quesenberry P, Sahoo S, Tahara H, Wauben MH, Witwer KW, Théry C.
J Extracell Vesicles. 2014 Dec 22;3:26913.
EVpedia: a community web portal for extracellular vesicles research.
Kim DK, Lee J, Kim SR, Choi DS, Yoon YJ, Kim JH, Go G, Nhung D, Hong K, Jang SC, Kim SH, Park KS, Kim OY, Park HT, Seo JH, Aikawa E, Baj-Krzyworzeka M, van Balkom BW, Belting M, Blanc L, Bond V, Bongiovanni A, Borràs FE, Buée L, Buzás EI, Cheng L, Clayton A, Cocucci E, Dela Cruz CS, Desiderio DM, Di Vizio D, Ekström K, Falcon-Perez JM, Gardiner C, Giebel B, Greening DW, Gross JC, Gupta D, Hendrix A, Hill AF, Hill MM, Nolte-'t Hoen E, Hwang do W, Inal J, Jagannadham MV, Jayachandran M, Jee YK, Jørgensen M, Kim KP, Kim YK, Kislinger T, Lässer C, Lee DS, Lee H, van Leeuwen J, Lener T, Liu ML, Lötvall J, Marcilla A, Mathivanan S, Möller A, Morhayim J, Mullier F, Nazarenko I, Nieuwland R, Nunes DN, Pang K, Park J, Patel T, Pocsfalvi G, Del Portillo H, Putz U, Ramirez MI, Rodrigues ML, Roh TY, Royo F, Sahoo S, Schiffelers R, Sharma S, Siljander P, Simpson RJ, Soekmadji C, Stahl P, Stensballe A, Stępień E, Tahara H, Trummer A, Valadi H, Vella LJ, Wai SN, Witwer K, Yáñez-Mó M, Youn H, Zeidler R, Gho YS.
Bioinformatics. 2015 Mar 15;31(6):933-9. doi: 10.1093/bioinformatics/btu741. Epub 2014 Nov 10.
Critical role of extracellular vesicles in modulating the cellular effects of cytokines.
Szabó GT, Tarr B, Pálóczi K, Éder K, Lajkó E, Kittel Á, Tóth S, György B, Pásztói M, Németh A, Osteikoetxea X, Pállinger É, Falus A, Szabó-Taylor K, Buzás EI.
Cell Mol Life Sci. 2014 Oct;71(20):4055-67. doi: 10.1007/s00018-014-1618-z. Epub 2014 Apr 6.
2016
2015
The Emerging and Diverse Roles of Src-Like Adaptor Proteins in Health and Disease.
Marton N, Baricza E, Érsek B, Buzás EI, Nagy G.
Mediators Inflamm. 2015;2015:952536. doi: 10.1155/2015/952536. Epub 2015 Aug 3. Review.
Genetic and epigenetic trends in telomere research: a novel way in immunoepigenetics.
Melicher D, Buzas EI, Falus A.
Cell Mol Life Sci. 2015 Nov;72(21):4095-109. doi: 10.1007/s00018-015-1991-2. Epub 2015 Jul 20.
Protein Tyrosine Phosphatase Nonreceptor Type 2: An Important Regulator of lnterleukin-6 Production in Rheumatoid Arthritis Synovial Fibroblasts.
Aradi B, Kato M, Filkova M, Karouzakis E, Klein K, Scharl M, Kolling C, Michel BA, Gay RE, Buzas EI, Gay S, Jüngel A.
Arthritis Rheumatol. 2015 Oct;67(10):2624-33. doi: 10.1002/art.39256.
Novel genes in Human Asthma Based on a Mouse Model of Allergic Airway Inflammation and Human Investigations.
Temesi G, Virág V, Hadadi E, Ungvári I, Fodor LE, Bikov A, Nagy A, Gálffy G, Tamási L, Horváth I, Kiss A, Hullám G, Gézsi A, Sárközy P, Antal P, Buzás E, Szalai C.
Allergy Asthma Immunol Res. 2014 Nov;6(6):496-503. doi: 10.4168/aair.2014.6.6.496. Epub 2014 Aug 19.
Myeloid-derived microRNAs, miR-223, miR27a, and miR-652, are dominant players in myeloid regulation.
Gilicze AB, Wiener Z, Tóth S, Buzás E, Pállinger É, Falcone FH, Falus A.
Biomed Res Int. 2014;2014:870267. doi: 10.1155/2014/870267. Epub 2014 Aug 11. Review.
Immunosuppressants increase the levels of natural autoantibodies reactive with glycosaminoglycans in myasthenia gravis.
Pal Z, Tóthfalusi L, Lörincz Z, György B, Molnar MJ, Falus A, Buzás EI.
J Neuroimmunol. 2014 Nov 15;276(1-2):224-8. doi: 10.1016/j.jneuroim.2014.08.002. Epub 2014 Aug 8.
Bayesian systems-based genetic association analysis with effect strength estimation and omic wide interpretation: a case study in rheumatoid arthritis.
Hullám G, Gézsi A, Millinghoffer A, Sárközy P, Bolgár B, Srivastava SK, Pál Z, Buzás EI, Antal P.
Methods Mol Biol. 2014;1142:143-76. doi: 10.1007/978-1-4939-0404-4_14. Review.