About the group


Main research interests of the group:

Investigations of phylogeny and ontogeny of chemotactic signalling. Drug-targeting. Evaluation of cell adhesion and migration in real-time, impedance based systems. Mathematical modelling of chemotaxis and chemotaxis assays.

Unicellular and mammalian cell culture models are used to investigate the optimal composition of chemotactic ligands in complex experiments focused on detailed description of 'Chemotaxome'. Assays of cell physiology and molecular genetics are applied to describe special receptor dynamics of chemotactic selection.

An intensive research is going on to improve theory and practice of „chemotactic drug targeting", and its clinico-pharmacology. Libraries of new anti-tumor conjugates are designed and tested. Tuftsin based oligopeptides furnished with fMLF chemoattractant moieties and methotrexate drug and GnRH based conjugates are used to characterize their cell physiological effects (e.g. chemotaxis, cell adhesion, phagocytosis, proliferation) and signalling mechanisms (e.g. receptor inhibition, IP3K blocking) in leukemic THP-1 monocyte and Mono Mac 6 monocyte models.

Analysis of cell adhesion - as the introductory phase of chemotaxis - is carried out by state of art impedance based techniques (ECIS and xCELLigence). The Research Group is the reference laboratory of Applied BioPhysics (USA), ibidi (Germany) and Roche-Hungary in Hungary. The mathematical routine developed by the Group makes possible to evaluate quantitatively even the micro motion of cells. The new ECIS Theta equipment working on 12 frequency ranges and measuring ohmic resistance (R), impedance (Z) and capacitance (C) provides the possibility to distinguish the number of adherent cells as well as their barrier function. A special wound healing assay is available to monitor migration of cells. xCELLigence SP and DP techniques are ideal complements of the above mentioned facilities - they are dedicated techniques of real time monitoring of cell adhesion and real chemotaxis even in low density cultures. In the frame of these experiments the group has an intensive work on design of novel chips adaptable into the equipments mentioned above.

The most recent trend of our research is to describe the molecular and cell biological backgrounds of beneficial effects of light treatment, described in clinical fields for decades. The Research Group has designed novel equipment,Cell-LED and a software controlling the lighting routines.


In Hungary:

  • ELTE-MTA Peptidkémiai Kutatócsoport, Budapest
  • MTA MFA KI MEMS Laboratórium, Budapest
  • SE, Gyógyszerhatástani Intézet Budapest
  • SE, Szerves Vegytani Intézet, Budapest
  • SE, Gyógyszertani Intézet, Budapest
  • SzTE, Szent-Györgyi Albert Klinikai Központ, Neurológiai Klinika, Szeged
  • SE, Parodontologiai Klinika, Budapest
  • SE, Oralbiológiai Intézet, Budapest
  • SE Testnevelési és Sporttudományi Tanszék, Budapest
  • SE, Kardiológiai Központ, Budapest
  • PTE, Szívgyógyászati Klinika, Pécs
  • Bay Zoltán Alkalmazott Kutatási Közhasznú Nonprofit Kft.



  • Dept. of Mechanical Engineering & Mechanics, School of Biomedical Engineering, Science & Health Systems, Drexel University, Philadelphia, USA
  • Department of Functional Bioscience, Gifu University School of Medicine, Gifu, JAPAN
  • Institut für Analytische Chemie, Chemo- & Biosensorik, Universität Regensburg
  • Johns Hopkins SOM, Department Pathology, Division Medical Microbiology, Baltimore, USA
  • Department of Pharmacology and Toxicology, Institute of Biomedicine, Faculty of Medicine, University of Oulu, Oulu, Finland
  • Department of Etology, Ecology and Evolution, University of Pisa, Pisa, Italy
  • Laboratorio de Fisiología Molecular, Instituto de Medicina Experimental, Escuela Luis Razetti, Facultad de Medicina, Universidad Central de Venezuela, Caracas, Venezuela
  • Faculty of Dental Medicine, Dept. Of Biochemistry, Carol Davila University, Bucharest, Romania
  • Laboratory of Analytical Chemistry and Biopolymer Structure Analysis, Dept. of Chemistry, University of Konstanz, Konstanz, Germany




  Kohidai Laszlo

Dr. László Kőhidai, Associate Professor



Dr. Orsolya Láng, Associate Professor

  Lajko Eszter

Dr. Eszter Lajkó, Senior Research Fellow



Dr. Éva Pállinger, Associate Professor

  no profile image

Adrienn Zsófia Szász, PhD Student

  Takacs Angela Angéla Takács, PhD Student

Research students:

  • Zsombor Kovács (SE, GYTK)
  • Máté Krausz (SE, ÁOK) 
  • Borbála Mádi (SE, ÁOK)
  • Gergely Orbán (SE, FOK)


  • Hamada, Michika
  • Milenova, Plamena
  • Ranganathan, Kiran
  • Samotik, Pawel
  • Szép Johanna
  • Tziakouri, Andria



  1. Kim D H, Casale D, Kőhidai L, Kim M J. 
    Galvanotactic and phototactic control of Tetrahymena pyriformis as a microfluidic workhorse. 
    Applied Physics Lett. 94, 16901-16903. (2009)  IF: 3.554
  2. Szabó R., Bánóczi Z., Mező G., Láng O., Kőhidai L., Hudecz F. 
    Daunomycin-polypeptide conjugates with antitumor activity.
    Biochim Biophys Acta. 1798, 2209-2216 (2010)  IF:4.180
  3. Kim, D.H., Cheang, U.K., Kőhidai, L., Byun, D., Kim, M.J. 
    Artificial magnetotactic motion control of Tetrahymena pyriformis using ferromagnetic nanoparticles A tool for fabrication of microbiorobots. 
    Applied Physics Lett. 97, 173702-173802-3  (2010) IF: 3.554
  4. Láng, O., Illyés, E., Menyhárd D.K., Láng, J., Sebestyén, F., Hudecz, F., Kőhidai, L. Chemotaxis induced by SXWS tetrapeptides in Tetrahymena-overlapping chemotactic effects of SXWS sequences and their identical amino acids. J. Mol. Recogn.Online 24-31 (2011) [IF: 2.959]
  5. Lajkó, E. Polgár, L., Lengyel, J., Láng, O., Kőhidai, L., Magyar, K. Basic cell physiological activities (cell adhesion, chemotaxis, proliferation) induced by selegiline and its derivatives in Mono Mac 6 human monocytes. J. Neural Transm - Online First [IF: 2.597]
  6. Sáfár, O., Kőhidai, L., Hegedűs, A. Time-delayed model of unibased movement of Tetrahymena pyriformis. Period. Math. Hung. 63, 215-225 /2011/ [IF: 0.394]
  7. Láng, J., Rákász, V., Magyar, A., Pállinger, É., Kőhidai, L. Chemotactic effect of odorants and tastants on the ciliate Tetrahymena pyriformis. J. Rec. Sign. Transd. 31, 423-433 /2011/ [IF: 1.822]
  8. Leurs, U., Lajkó, E.,Mező, G., Orbán, E., Öhlschläger, C.P., Marquardt, A., Kőhidai, L., Manea, M. GnRH-III based multifunctional drug delivery systems containing daunorubicin and methotrexate. Eur. J. Med. Chem. (Online) [IF: 3.193]
  9. Kohidai, L. Tetrahymena pyriformis in motion. In: Microbiorobotics - Biologically Inspired Microscale Robotic Systems. Eds. Kim, MJ, Steager, E., Agung, AJ. Elsevier, pp 55-84 /2012/
  10. Arany E, Láng J, Somogyvári D, Láng O, Alapi T, Ilisz I, Gajda-Schrantz K, Dombi A, Kőhidai L, Hernádi K. Vacuum ultraviolet photolysis of diclofenac and the effects of its treated aqueous solutions on the proliferation and migratory responses of Tetrahymena pyriformis.Sci Total Environ. 468-469C, 996-1006 /2013/ [IF: 3.163]
  11. Díaz E, Köhidai L, Ríos A, Vanegas O, Silva A, Szabó R, Mező G, Hudecz F, Ponte-Sucre A. Leishmania braziliensis: Cytotoxic, cytostatic and chemotactic effects of poly-lysine-methotrexate-conjugates. Exp Parasitol. 135, 134-141 /2013/ [IF: 1.859]
  12. Lajkó E, Szabó I, Andódy K, Pungor A, Mező G, Kőhidai L. Investigation on chemotactic drug targeting (chemotaxis and adhesion) inducer effect of GnRH-III derivatives in Tetrahymena and human leukemia cell line.J Pept Sci. 19, 46-58 /2013/ [IF: 1.862]
  13. Kőhidai L, Lajkó E, Szabó I, Polgár I, Manea M, Mező G. GnRH-based drug targeting: Cell adhesion and migration modulator effects of GnRH derivatives in tumor cells. In: Gonadotropin-releasing hormone (GnRH): Production, structure and functions. Ed. Sills ES; Nova Publ. pp. 254-272 /2013/
  14. Abdallah, M.W., Michel, T., Kohidai, L. Autism spectrum disorders and circulating chemokines. In: The Comprehensive Guide to Autism, Eds. Patel, VB, Preedy, VR., Martin, CR; Springer; pp 1627-1642 /2014/
  15. Kuo CH, Láng J, Láng O, Kőhidai L, Sivaniah E. The facile generation of two-dimensional stiffness maps in durotactic cell platforms through thickness projections of three-dimensional submerged topography. Methods Cell Biol. 121:49-60 /2014/ [IF: 1.440]
  16. Diaz E, Zacarias AK, Pérez S, Vanegas O, Köhidai L, Padrón-Nieves M, Ponte-Sucre A. Effect of aliphatic, monocarboxylic, dicarboxylic, heterocyclic and sulphur-containing amino acids on Leishmania spp. chemotaxis. Parasitology 23:1-10 /2015/ [IF: 2.560]
  17. Szemes Á, Lajkó E, Láng O, Kőhidai L. Chemotactic effect of mono- and disaccharides on the unicellular Tetrahymena pyriformis. Carbohydrate Research 407:158-165 /2015/ [IF: 1.929]
  18. Enyedi KN, Czajlik A, Knapp K, Láng A, Majer Z, Lajkó E, Kőhidai L, Perczel A, Mező G. Development of Cyclic NGR Peptides with Thioether Linkage: Structure and Dynamics Determining Deamidation and Bioactivity. J Med Chem. 58(4):1806-1817 /2015/ [IF: 5.480]
  19. Muto Y, Guindon S, Umemura T, Kőhidai L, Ueda H. Adaptive evolution of formyl Peptide receptors in mammals. J Mol Evol. 80:130-141 /2015/ [IF: 1.863]
  20. Szabó, R., Láng, O., Láng, J., Illyés, E., Kőhidai, L. Hudecz, F. Effect of SXWS/WSXWS peptides on chemotaxis and adhesion of the macrophage-like cell line J774. J Mol Recogn. 28(4):253-260 /2015/ [IF: 2.337]
  21. Kőhidai L, Tóth K, Samotik P, Ranganathan K, Láng O, Tóth M, Ruskoaho H. Effect of vasoactive peptides in Tetrahymena - Chemotactic activities of adrenomedullin, proadrenomedullin N-terminal 20 peptide (PAMP) and calcitonin gene-related peptide (CGRP). Mol. Cell. Biochem. 411(1-2):271-80. /2016/ [IF: 2.613]
  22. Lajkó E, Bányai P, Zámbó Zs, Krusinszki L, Szőke É, Kőhidai L. Targeted tumor therapy by Rubia tinctorum L.: analytical characterization of hydroxyanthraquinones and investigation of their selective cytotoxic, adhesion and migration modulator effects on melanoma cell lines (A2058 and HT168-M1). Cancer Cell Internat. 15:119 /2015/ [IF: 2.884]
  23. Biri B, Kiss B, Király R, Schlosser G, Láng O, Kőhidai L, Fésüs L, Nyitray L. Metastasis-associated S100A4 is a specific amine donor and an activity-independent binding partner of transglutaminase-2. Biochemical J. 473:31-42 /2016/ [IF: 3.562]
  24. Kőhidai L. Chemotaxis as an expression of communication of Tetrahymena. In: Biocommunication of ciliates. Ed: Witzany G. Springer pp. 65-82 /2016/
  25. Becker H., Soós P., Lajkó E., Láng O., Merkely B., Szabó G., Dohmen P.M., Weymann A., Kőhidai L. Impedimetric Analysis of the Effect of Decellularized Porcine Heart Scaffold on Human Fibrosarcoma, Endothelial, and Cardiomyocyte Cell Lines. Medical Science Monitor doi:10.12659/MSM.901527. (2017)





Invited Lectures

  • Kőhidai, L. Chemotaxome – A new member with complex biological entities in systems biology. (BSS Formal Seminar, Cavendish Laboratory, University of Cambridge, 2013.)
  • Kőhidai, L. ECIS Migration (ECIS Tutorial on Impedance-Based Cellular Assays, Budapest, 2013.)
  • Kőhidai, L. Chemotaxome (158th iCeMS Seminar - iCeMS - Kyoto University, Kyoto, 2014)
  • Kőhidai, L. "Chemotaxome" - A kemotaxis új, rendszerbiológiai alapú szemlélete. (Semmelweis Genomikai Hálózat, 15. évfolyam / 138. rendezvény, Budapest, 2015)


PhD Scientific Days 2013

Budapest, 2013

  • Polgár, L. Measurement of platelet spreading and adhesion on human platelets with impedimetry.
  • Lajkó, E., Pállinger, É., Csaba, G., Kőhidai, L. The long term effect of hormonal imprinting induced by insulin and serotonin on cell physiological parameters of Tetrahymena.
  • Slezák, S., Lajkó, E., Pállinger, É., Bai, K.B., Mező, G., Kőhidai, L. Monitoring of the adhesion of crevicular fluid cells and its modification by oligotuftsin derivatives. 
  • Lajkó, E., Bányai, P., Szőke, É., Kőhidai, L. Anthraquinone components of Rubia tinctorum as candidate anticancer agents for drug targeting.


MTA Peptidkémiai Munkabizottság Ülése

Balatonszemes 2013

  • Szabó, I., Bősze, Sz., Lajkó, E., Kőhidai, L., Mező, G. Antiadhezív aktivitású GnRH konjugátumok tervezése és szintézise.
  • Lajkó, E., Szabó, I., Mező, G., Kőhidai, L. Fibronektin eredetű peptidfragmenst tartalmazó GnRH konjugátumok hatása melanóma sejtek adhéziós viselkedésére.
  • Láng, J., Pomázi, K., Láng, O., Mező, G., Kőhidai, L. W-S-EWS peptidek és OT20 konjugátumaik hatása tumoros sejtvonalak migrációjára, adhéziójára és immunfenotípusára.
  • Kőhidai, L., Csercsik, R., Samotik, P., Ranganathan, K., Banchetti, R., Vallesi, A., Láng, O. Protozoon feromonok sejtélettani hatásai - Fajspecifikusan hat-e minden feromon?


2nd Conference on Impedance-Based Cellular Assays - IBCA2013

Budapest, 2013

  • Kőhidai, L., Láng, O., Lajkó, E., Láng, J., Polgár, L., Mező, G. Impedimetry assays – Dedicated ways to evaluate migratory behavior of cells
  • Láng, O., Nagy, K., Láng, J., Perczel-Kovách, K., Varga, G., Kőhidai, L. Monitoring dental stem cells on self-assembling peptide hydrogel scaffold by impedance based techniques.
  • Lajkó, E., Bányai, P., Zámbó, Zs., Szőke, É., Kőhidai, L. Natural cancer therapeutics – The effects of anthraquinones derived from Rubia tinctorum L. on melanoma cell lines.
  • Kosztin, A., Polgár, L., Kőhidai, L., Földes, G. Angiogenic effect of human pluripotent stem cell-derived endothelial cells.
  • Polgár, L., Soós, P., Lajkó, E., Láng, O., Merkely, B., Kőhidai, L. Measurement of platelet function with impedimetry - The effect of agonists and heparin on adhesion and spreading of human platelets.
  • Lajkó, E., Bányai, P., Zámbó, Zs., Szőke, É., Kőhidai, L. Natural cancer therapeutics – The effects of anthraquinones derived from Rubia tinctorum L. on melanoma cell lines.
  • Kosztin, A., Polgár, L., Kőhidai, L., Földes, G. Angiogenic effect of human pluripotent stem cell-derived endothelial cells.
  • Polgár, L., Soós, P., Lajkó, E., Láng, O., Merkely, B., Kőhidai, L. Measurement of platelet function with impedimetry - The effect of agonists and heparin on adhesion and spreading of human platelets.
  • Biri, B., Láng, O., Méhes, E., Czirók, A., Kőhidai, L., Nyitray, L. Motility and adhesion assays related to the effect of metastasis-associated S100A4 protein.
  • Láng, J., Kuo, R., Láng, O., Kőhidai, L., Sivaniah, E. Study of the interdependence of cell adhesion migration and mechanosensing using a complex, innovative approach.
  • Slezák, S., Lajkó, E., Pállinger, É., Bai, K.B., Mező, G., Gera, I., Kőhidai, L. Monitoring of the effect of oligotuftsin derivatives on the adhesion of crevicular fluid cells.
  • Lajkó, E., Mércz, K., Manea, M., Mező, G., Kőhidai, L., Pállinger, É.  Cell adhesion and proliferation of BeWo choriocarcinoma cell line induced by GnRH-III based targeted systems. 
  • Bäcker, H., Polgár, L., Soós, P., Lajkó, E., Láng, O., Weymann, A., Szabó, G., Kőhidai, L. Effect of heart scaffold ECM proteins on cell adhesion of cardiac myocytes - Model experiments of cell adhesion based loading of porcine heart scaffold
  • Németh, A., Bodor, Cs., Mirzahosseini, S., Kőhidai, L., Rosivall, L. Comparing the effects of VEGF and angiotensin II on endothelial permeability measured by ECIS and transwell assay.


MTA Peptidkémiai Munkabizottság Ülése

Balatonszemes 2014

  • Láng, O., Nagy, K., Perczel-Kovách, K., Láng, J., Fülöp, L., Varga, G., Kőhidai, L. Emberi fog eredetű őssejtek vizsgálata peptid alapú hidrogéleken.
  • Lajkó, E., Zámbó, Zs., Kollár, Gy., Kőhidai, L. L-karnozin dipeptid sejtélettani folyamatokra kifejtett hatásának vizsgálata csillós egysejtű és humán melanóma modell-sejteken.
  • Kőhidai, L., Mércz, K., Pállinger, É., Lajkó, E. Gyógyszer célbajuttatásra tervezett, GnRH alapú konjugátumok sejtélettani hatásainak vizsgálata BeWo choriocarcinoma sejtvonalon.


MTA Peptidkémiai Munkabizottság Ülése

Balatonszemes, 2015

  • Pethő, L., Láng, O., Kasza, Gy., Kőhidai, L., Mező, G. EGF receptort célzó konjugátumok szintézise és biológiai aktivitása.
  • Enyedi, K.N., Láng, A., Czajlik, A., Knapp, K., Majer, Zs., Lajkó, E., Kőhidai, L., Tóth, Sz., Szakács, G., Perczel, A., Mező, G. Egy anyag két célpont. Lehetőségek az irányított tumorterápiában.


Hungarian Molecular Life Sciences Conference 2015

Eger, Hungary, 2015

  • Láng, O., Láng, J., Sivaniah, E., Kőhidai, L. Mechanopharmacological screening platform.
  • Láng, O.,  Kőhidai, Zs.,  Nagy, K., Láng, J., Nagy, K., Perczel-Kovách, K.,  Lohinai, Zs., Varga, G., Kőhidai, L. Biological effect of mouthwash compounds on dental stem cells and gingival epithelium.
  • Biri, B., Kiss, B.,  Király, R., Schlosser, G., Láng, O., Kőhidai, L., Fésüs, L., Nyitray, L. Metastasis-associated S100A4 serves as a specific amine substrate for tissue transglutaminase-2. 


MTA Peptidkémiai Munkabizottság Ülése

Balatonszemes, 2016

  • Kőhidai, L., Hamada, M., Lajkó, E., Horváti, K., Mező, G. Muto, Y., Láng, O. A CCL2 kemokin molekuláris evolúciója - A GAG-kötő 89H, 47R és 72K pentapeptidek jelentőségének vizsgálata Tetrahymena modell-sejten
  • Lajkó, E., Law, J., Spring, S., Hempel, F., Mező, G., Ingebrandt, S., Kőhidai, L. GnRH-III alapú konjugátumok egysejt-szintű toxicitás vizsgálata field-effect transistor és microelectrode array alapú módszerekkel.

  • Láng, O., Debrei, D., Kőhidai, Zs., Komlósi, N., Slezák, S., Földes, A., Varga, G., Mező, G., Kőhidai, L. TKPPR alapú peptidkönyvtár vizsgálata szájüregi modellsejteken.

  • Khorolsuren, Z., Kőhidai, Zs., Földes, A., Varga, G., Mező, G., Láng, O., Vág, J., Kőhidai, L. Characterization of poly-L-lysine based synthetic polypeptide conjugates as potential dental therapeutic materials.

 3rd Conference on Impedance-Based Cellular Assays

Regensburg, 2016

  • Lajkó, E., Spring, S., Láng, O., Ingebrandt, S., Mező, G., Kőhidai, L. Impedance-based analysis and holographic phase imaging of the GnRH-III-based drug-targeting in melanoma cells.

  • Láng, O., Kőhidai, L. Impedimetric characterisation of cell physiological responses in oral biology.

  • Takács, A., Lajkó, E., Istenes, I., Kőhidai, L., Láng, O. Comparative study on the effectiveness of bortezomib in combination with alpha lipoic acid and thiamine in melanoma and myeloma cell lines. 

  • Lajkó, E., Spring, S., Láng, O., Ingebrandt, S., Mező, G., Kőhidai, L. Impedance-based analysis and holographic phase imaging of the GnRH-III-based drug-targeting in melanoma cells.


Other Scientific Meetings

  • Lajkó, E., Bányai, P., Szőke, É., Kőhidai, L. The anthraquinone components of Rubia tinctorum L are suitable anticancer drugs for application in targeted tumor therapy. (1st European PhD Conference, Budapest, 2013.)
  • Polgár, L. Measurement of the effect of decellularized porcine heart scaffold on the adhesion of human cardiovascular cell lines using impedimetric technique. (PhD Scientific Days 2014, Budapest, 2014)
  • Baricza, E., Molnár-Érsek, B., Lajkó, E., Kőhidai, L., Buzás, L., Nagy, Gy. The distinct regulation of interleukin-17 and interleukin-22 production during human TH17 cell differentiation. (MIT 43. Vándorgyűlése, Velence)
  • Biri, B., Lajkó, E., Láng, O., Kőhidai, L., Nyitray, L. The significance of the metastasis-associated S100A4 protein in cell adhesion and migration of A431 epithelial carcinoma cell line. (Annual Conference of the Hungarian Biochemical Society, Debrecen, 2014)
  • Kosztin, A., Polgár, L., Kőhidai, L., Várnai, P., Gara, E., Skopál, J., Harding, S., Merkely, B., Földes, G. Angiogenic and positive inotropic effects of apelin fragments on human pluripotein stem cell-derived cardivascular cells. (FEPS-2014, Budapest, 2014)
  • Lajkó, E., Mércz, K., Polgár, L., Manea, M., Szabó, I., Pállinger, É., Mező, G., Kőhidai, L. Investigations on the prospective use of GnRH-III based conjugates for targeted chemotherapy as drug delivery systems in different tumor cells. (The 11th International Symposium on GnRH, Salzburg, Austria, 2014)
  • Enyedi, K.N., Kőhidai, L., Lajkó, E., Mező, G. Targeting cancer-associated fibroblast by their tumor specific receptors. (EU COST, Chemical Approaches to Targeting Drug Resistance in Cancer Stem Cells - 2nd Working Group Meeting, Budapest, Hungary, 2014)
  • Istenes I., Lajkó, E., Takács, A., Láng, O., Kőhidai, L.,  Demeter, J., Nagy,  Zs. Alfa liponsav és bortezomib együttes in vitro adásának vizsgálata U266 myeloma sejtvonalon.  (Magyar Hematológiai és Transzfúziológiai Társaság 19. Lymphoma Konferencia, Tata, 2014)
  • Oláhné Szabó, R., Sebestyén, M., Kóczán, Gy., Kőhidai, L., Hudecz, L. Drug targeting strategy with polyeptide based methotrexate conjugates against Leishmania infection. (17th International Congress of the Hungarian Society for Microbiology, Budapest, 2015)
  • Kovács, Á.F., Láng, O., Kőhidai, L., Rigó, L., Fekete, N., Buzás, E.I., Pállinger, É. Impact of preeclampsia-associated extracellular vesicles on the monocyte-macrophage system: an in vitro analysis. (ISEV 2016 Annual Meeting, Rotterdam, 2016)



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