Topic list
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Lectures (Lecturer: Tóth S.) Tuesday 17.00-18.30, NET L16
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1st week
(12 Sept.)
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Timeline of epigenetics; levels of epigenetic changes; targets of epigenetics

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2nd week
(19 Sept.)
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DNA methylation; de novo and maintenance methylation; DNMT interactions

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3rd week
(26 Sept.)
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Histone modifications; histone code; histone modifying enzymes

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4th week
(03 Oct.)
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Citrullination; Methylated DNA binding proteins; post-transcriptional RNA modifications; epitranscriptomics

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5th week
(10 Oct.)
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Polycomb and trithorax complexes; Chromatin remodelling

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6th week
(17 Oct.)
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Non-coding RNAs in epigenetic regulation; Transposons; Paramutation, transvection

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7th week
(24 Oct.)
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Genome imprinting

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8th week
(31 Oct.)
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X inactivation and skewed X inactivation: their connection to DNA methylation

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9th week
(07 Nov.)
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Epigenetic changes during early development and gametogenesis; epigenetic consequences of cloning and ART

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10th week
(14 Nov.)
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Transgenerational epigenetics; epigenetics of endocrine disruptors; maternal behaviour and its epigenetic consequences

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11th week
(24 Nov.)
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Small molecules, clock genes and intermediate metabolism in epigenetic regulation

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12th week
(28 Nov.)
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Role of epigenetics in carcinogenesis; epigenetic therapy in cancer; Mitotic bookmarking

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13th week
(05 Dec.)
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Epigenetics in aging and autoimmunity

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14th week
(12 Dec.)
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Epigenetic changes in diseases of the central nervous system

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The exam grade will be based on a written test. The test questions will cover the topics of the lectures presentations.
Lecturer: Sara Tóth Phd, associate professor
NET 6th floor, room 619.
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.
Phone: 210-2630/56244
Keywords
- Definition of epigenetics; levels of epigenetic regulation related to transcription; types of epigenetic processes; mutual/putative aim of epigenesis
- DNA methylation; consequences of mC formation (nucleotide ratio, mutations, carcinogenesis); methyl donors; role of folate; CpG and CpG islands; CpG island and gene body and their methylation; DNMT1 and DNMT3, DNMT3L; alternative promoter use and alternative splicing in gametogenesis; MBD and MeCP2; Rett-syndrome; TET-enzymes, hydroxyl-methyl cytosine (5-hmC); 5-caC and 5-fC and their role in gene regulation
- Histone modifications; histone code; frequently modified amino acids of histones; HAT and HDAC; HMT and HDM; interactions between the modified histones; bivalent histone modification in ES cells; alternative histone variants
- Chromatin remodelling; polycomb and trithorax group of proteins; their interaction with modified histones and DNA; CTCF and cohesion and functional organization of chromatin, TADs
- Post-translational modifications; citrullination and disease; histone citrullination
- Post transcriptional RNA modifications; epitranscriptomics
- Types of non-coding RNAs; genomic localization; tsRNA, siRNA and RNAi; microRNAs (pri-miR and pre-RNA) and lncRNAs (Xist and lincRNA); gene silencing through these RNAs; types of interactions leading to gene expression modification
- Position effect; silencing of transposons and retroviral elements; IAP and agouti and yellow colour; food supplementation;
- Imprinting; parental conflict theory; IGF2 and IGF2R; bipedality as an evolutionary explanation; loss of imprinting (LOI); inprinting diseases; Prader-Willi and Angelman syndromes; differentially methylated regions (DMRs); Beckwith-Wiedemann and Silver-Russel syndromes; UDP as a cause of imprinting diseases; imprinting centres (ICs), insulators;
- X-chromosome inactivation; random and imprinted; dose compensatory mechanisms; XIC and XIST; counting and choice; Tsix; MSUD; skewed X inactivation and diseases/phenomena related to it; territorial arrangement of X inactivation; evolutionary and tissue specific differences in X inactivation; escape and escapees,; DNA methylation and characteristic histone modifications;
- Paramutation; transvection; mitotic bookmarking; RNA and protein transfer from parents to offspring;
- DNA methylation during the early stages of embryogenesis; pluripotency factors in X inactivation and in reprogramming; ES and iPS cells in epigenetic research; cloning; ART and increased Beckwith–Wiedemann birth rate; Transgenerational epigenesis; Dutch famine; role of diet, calory restriction, endocrine disruptors and maternal behaviour;
- Cancer epigenetics; CpG island hypermethylation; gene body hypomethylation; genetic and epigenetic interactions; genome instability; CpG island shore methylation; TSG and oncogene silencing and activation; cell cycle regulatory and DNA repair proteins as targets of epimutations; HDAC and DNMT inhibitors: problem of specificity and targeting resistance;
- Epigenetics of aging; telomeres and telomerase in aging; subtelomeric and telomeric epigenetic features; twin studies; aging regulatory network;
- Maternal behaviour and its transgenerational epigenetic consequences; mental diseases (ADHD, schizophrenia and psychosis) and epigenetics; social status, stress and their epigenetic effects;